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验证数据展示

  • WB analysis of HEK-293 using 22697-1-AP

    WB analysis of HEK-293 using 22697-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22697-1-AP (DOK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 22697-1-AP

    WB analysis of Jurkat using 22697-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22697-1-AP (DOK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skeletal muscle using 22697-1-AP

    IHC staining of human skeletal muscle using 22697-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22697-1-AP (DOK7 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skeletal muscle using 22697-1-AP

    IHC staining of human skeletal muscle using 22697-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22697-1-AP (DOK7 Antibody) at dilution of 1:50 (under 40x lens).

DOK7 Polyclonal antibody

DOK7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22697-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C4orf25, CMS1B, docking protein 7, Dok 7, DOK7, Protein Dok 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, MCF-7 cells, HEK-293 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22697-1-AP targets DOK7 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 DOK7 fusion protein Ag18543 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 docking protein 7
别名 C4orf25, CMS1B, docking protein 7, Dok 7, DOK7, Protein Dok 7
计算分子量 504 aa, 53 kDa
观测分子量 64 kDa
GenBank蛋白编号BC131544
基因名称 DOK7
Gene ID (NCBI) 285489
RRIDAB_2879152
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ18PE1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOK7, also named as C4orf25, is a MuSK-interacting cytoplasmic protein. DOK7 is essential for neuromuscular synaptogenesis through its interaction with MuSK. It plays an essential role in neuromuscular synaptogenesis. DOK7 acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. DOK7 has some isoforms with MW 53 kDa, 37 kDa, 28 kDa and 64 kDa.

实验方案

Product Specific Protocols
WB protocol for DOK7 antibody 22697-1-APDownload protocol
IHC protocol for DOK7 antibody 22697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols