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验证数据展示

  • WB analysis using 31993-1-AP

    WB analysis using 31993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31993-1-AP (DOCK11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 31993-1-AP

    IHC staining of mouse kidney using 31993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31993-1-AP (DOCK11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DOCK11 antibody included
  • IHC staining of mouse kidney using 31993-1-AP

    IHC staining of mouse kidney using 31993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31993-1-AP (DOCK11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DOCK11 antibody included

DOCK11 Polyclonal antibody

DOCK11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31993-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Zizimin 2, ZIZ2, dedicator of cytokinesis 11, bB128O4.1, ACG

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, RAW 264.7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DOCK11 Ready-To-Use IHC Kit. DOCK11 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31993-1-AP targets DOCK11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35831

Product name: Recombinant human DOCK11 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 250-400 aa of NM_144658

Sequence: HYLAAETEQEMEEWLITLKKIIQINTDSLVQEKKETVETAQDDETSSQGKAENIMASLERSMHPELMKYGRETEQLNKLSRGDGRQNLFSFDSEVQRLDFSGIEPDIKPFEEKCNKRFLVNCHDLTFNILGQIGDNAKGPPTNVEPFFINL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dedicator of cytokinesis 11
别名 Zizimin 2, ZIZ2, dedicator of cytokinesis 11, bB128O4.1, ACG
计算分子量238 kDa
观测分子量238 kDa
GenBank蛋白编号NM_144658
基因名称 DOCK11
Gene ID (NCBI) 139818
RRIDAB_3670169
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5JSL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DOCK11(also known as Zizimin2) is a member of CDM (Ced-5, DOCK180, and Myoblast City) family guanine nucleotide exchange factor mainly expressed in immune cells. As a guanine nucleotide exchange factor, DOCK11 activated the rho family GTPase cell division cycle 42 (CDC42), resulting in cytoskeletal reorganization.

实验方案

Product Specific Protocols
IHC protocol for DOCK11 antibody 31993-1-APDownload protocol
WB protocol for DOCK11 antibody 31993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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