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验证数据展示

  • WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

    WB analysis using 20954-1-AP (same clone as 20954-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20954-1-AP (DNMT3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 20954-1-AP (same clone as 20954-1-PBS)

    IF Staining of K-562 using 20954-1-AP (same clone as 20954-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 20954-1-AP (same clone as 20954-1-PBS)

    WB analysis of HepG2 using 20954-1-AP (same clone as 20954-1-PBS)

    WB result of DNMT3A antibody (20954-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNMT3A transfected HepG2 cells. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)

    IHC staining of mouse testis using 20954-1-AP (same clone as 20954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20954-1-AP (DNMT3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DNMT3A antibody included
  • IP experiment of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    IP experiment of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    IP result of anti-DNMT3A (IP:20954-1-AP, 4ug; Detection:20954-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    IF Staining of HeLa using 20954-1-AP (same clone as 20954-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNMT3A antibody (20954-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20954-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DNMT3A Polyclonal antibody, PBS Only

DNMT3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20954-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

M.HsaIIIA, EC:2.1.1.37, EC:2.1.1.-, DNA MTase HsaIIIA, DNA (cytosine-5)-methyltransferase 3A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DNMT3A Ready-To-Use IHC Kit. DNMT3A primary antibody included.

产品信息

20954-1-PBS targets DNMT3A in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNMT3A fusion protein Ag15111 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA (cytosine-5-)-methyltransferase 3 alpha
别名 M.HsaIIIA, EC:2.1.1.37, EC:2.1.1.-, DNA MTase HsaIIIA, DNA (cytosine-5)-methyltransferase 3A
计算分子量 912 aa, 102 kDa
观测分子量 120-130 kDa
GenBank蛋白编号BC043617
基因名称 DNMT3A
Gene ID (NCBI) 1788
RRIDAB_10733339
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6K1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.