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验证数据展示

  • WB analysis using 26721-1-AP (same clone as 26721-1-PBS)

    WB analysis using 26721-1-AP (same clone as 26721-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26721-1-AP (DNAJC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 26721-1-AP (same clone as 26721-1-PBS)

    IHC staining of human colon cancer using 26721-1-AP (same clone as 26721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 26721-1-AP (same clone as 26721-1-PBS)

    IF Staining of U2OS using 26721-1-AP (same clone as 26721-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 26721-1-AP (DNAJC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

DNAJC3 Polyclonal antibody, PBS Only

DNAJC3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 26721-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

26721-1-PBS targets DNAJC3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24966

Product name: Recombinant human DNAJC3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 32-380 aa of BC047936

Sequence: GVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDNYIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSENEEKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKILEVCVWDAELRELRAECFIKEGEPRKAISDLKAASKLKNDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIRDGRYTDATSKYESVMKTEPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAYLIEEMYDEAIQDYETAQEHNENDQQIREGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C, member 3
别名
观测分子量58 kDa
GenBank蛋白编号BC047936
基因名称 DNAJC3
Gene ID (NCBI) 5611
RRIDAB_2880613
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13217
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

DNAJC3, also known as P58,HP58 and P58IPK, belongs to heat shock protein family,which is a kind of conservative molecules that expresses extensively in cells of human and animals and protects cells against all kinds of damage. As a cochaperone of BiP that an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum(ER), DNAJC3 plays a crucial role in ER protein folding and the UPR(unfolded protein response) (PMID:10597629; PMID:25466870). Studies reported that DNAJC3 related several diseases such as osteosarcoma, widespread neurodegeneration, diabetes mellitus, sensorimotor neuropathy, ataxia, sensorineural hearing loss and short body stature(PMID:30652414; PMID:28940199).

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