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验证数据展示

  • WB analysis using 26229-1-AP

    WB analysis using 26229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26229-1-AP (DNAJC25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 26229-1-AP

    IHC staining of human liver using 26229-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26229-1-AP (DNAJC25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 26229-1-AP

    IHC staining of human liver using 26229-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26229-1-AP (DNAJC25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 26229-1-AP

    IF Staining of U2OS using 26229-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC25 antibody (26229-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

DNAJC25 Polyclonal antibody

DNAJC25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26229-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

DNAJC25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human placenta tissue, mouse liver tisue, rat liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26229-1-AP targets DNAJC25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DNAJC25 fusion protein Ag23760 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DnaJ (Hsp40) homolog, subfamily C , member 25
别名 DNAJC25
观测分子量36-42 kDa
GenBank蛋白编号BC048318
基因名称 DNAJC25
Gene ID (NCBI) 548645
RRIDAB_3085849
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1X3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DnaJ heat shock protein family (Hsp40) member C25 (DNAJC25), which belongs to the HSP40 subfamily C, is localized to the cytoplasm. DNAJC25 had a markedly high expression level in liver tissues and was significantly downregulated in liver cancer tissues compared with the adjacent normal tissues (PMID: 23205125).

实验方案

Product Specific Protocols
WB protocol for DNAJC25 antibody 26229-1-APDownload protocol
IHC protocol for DNAJC25 antibody 26229-1-APDownload protocol
IF protocol for DNAJC25 antibody 26229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols