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验证数据展示

  • IHC staining of human lung using 31079-1-AP (same clone as 31079-1-PBS)

    IHC staining of human lung using 31079-1-AP (same clone as 31079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31079-1-AP (DNAH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 31079-1-AP (same clone as 31079-1-PBS)

    IHC staining of mouse lung using 31079-1-AP (same clone as 31079-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31079-1-AP (DNAH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 31079-1-AP (same clone as 31079-1-PBS)

    IF Staining of HeLa using 31079-1-AP (same clone as 31079-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNAH5 antibody (31079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 31079-1-AP (same clone as 31079-1-PBS)

    IF Staining of HepG2 using 31079-1-AP (same clone as 31079-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAH5 antibody (31079-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

DNAH5 Polyclonal antibody, PBS Only

DNAH5 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 31079-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

KIAA1603, HL1, Dynein heavy chain 5, axonemal, DNAHC5, Ciliary dynein heavy chain 5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

31079-1-PBS targets DNAH5 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag34647

Product name: Recombinant human DNAH5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-150 aa of NM_001369

Sequence: LNKTEVEDAILEGNQIERIDQLFAVGGLRHLMFYYQDVEEAETGQLGSLGGVNLVSGKIKKPKVFVTEGNDVALTGVCVFFIRTDPSKAITPDNIHQEVSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 dynein, axonemal, heavy chain 5
别名 KIAA1603, HL1, Dynein heavy chain 5, axonemal, DNAHC5, Ciliary dynein heavy chain 5
计算分子量529 kDa
GenBank蛋白编号NM_001369
基因名称 DNAH5
Gene ID (NCBI) 1767
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8TE73
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dynein axonemal heavy chain 5 (DNAH5) is an axonemal heavy chain dynein, part of a microtubule-associated motor protein complex. DNAH5 is a large gene comprising 79 exons and one alternative first exon and encodes a heavy chain of the ODA. We have recently shown that recessive DNAH5 mutations are responsible for PCD characterized by ODA defects. DNAH5 mutations cause PCD and randomization of left-right asymmetry (PMID: 15750039).

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