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验证数据展示

  • IHC staining of human spleen using 15460-1-AP

    IHC staining of human spleen using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with DLC1 antibody included
  • IHC staining of human spleen using 15460-1-AP

    IHC staining of human spleen using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with DLC1 antibody included
  • IF Staining of HepG2 using 15460-1-AP

    IF Staining of HepG2 using 15460-1-AP

    Immunofluorescent analysis of HepG2 cells, using DLC1 antibody 15460-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse lung using 15460-1-AP

    IP experiment of mouse lung using 15460-1-AP

    IP result of anti-DLC1 (IP:15460-1-AP, 4ug; Detection:15460-1-AP 1:300) with mouse lung tissue lysate 2400ug.

  • IHC staining of mouse small intestine using 15460-1-AP

    IHC staining of mouse small intestine using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15460-1-AP (DLC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLC1 antibody included
  • IHC staining of human placenta using 15460-1-AP

    IHC staining of human placenta using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15460-1-AP (DLC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLC1 antibody included
  • IHC staining of mouse kidney using 15460-1-AP

    IHC staining of mouse kidney using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15460-1-AP (DLC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLC1 antibody included
  • IHC staining of mouse ovary using 15460-1-AP

    IHC staining of mouse ovary using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15460-1-AP (DLC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DLC1 antibody included

DLC1 Polyclonal antibody

DLC1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Cat No. 15460-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, IP, ELISA and More (2)

别名

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC-1, HP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inmouse lung tissue
Positive IHC detected inhuman spleen tissue, human placenta tissue, human liver tissue, mouse small intestine tissue, mouse kidney tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DLC1 Ready-To-Use IHC Kit. DLC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15460-1-AP targets DLC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, rat
免疫原

CatNo: Ag7732

Product name: Recombinant human DLC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1179-1528 aa of BC054511

Sequence: KDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSADYQHFLQDCVDGLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRSVIEVPAVPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVDLRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 deleted in liver cancer 1
别名 ARHGAP7, deleted in liver cancer 1, DLC 1, DLC-1, HP
计算分子量 171 kDa
GenBank蛋白编号BC054511
基因名称 DLC1
Gene ID (NCBI) 10395
RRIDAB_2246077
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96QB1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for DLC1 antibody 15460-1-APDownload protocol
IHC protocol for DLC1 antibody 15460-1-APDownload protocol
IP protocol for DLC1 antibody 15460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Res

Mutant KRAS and CK2 Cooperatively Stimulate SLC16A3 Activity to Drive Intrahepatic Cholangiocarcinoma Progression

Authors - Ran Chen
View Article
humanWB

Biomaterials

Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs

Authors - Anna L Kersey
View Article
ratWB,IHC

J Sex Med

Hypoandrogenic state inhibits erectile function in rats via miR-200a-3p

Authors - Yuan Wang
View Article
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