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DLC1 Polyclonal antibody

DLC1 Polyclonal Antibody for IF/ICC, IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP,ELISA

Conjugate

Unconjugated

Cat No : 15460-1-AP

Print datasheet

Synonyms

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12

验证数据展示

  • IHC staining of human spleen using 15460-1-AP

    IHC staining of human spleen using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 15460-1-AP

    IHC staining of human spleen using 15460-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 15460-1-AP

    IF Staining of HepG2 using 15460-1-AP

    Immunofluorescent analysis of HepG2 cells, using DLC1 antibody 15460-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse lung using 15460-1-AP

    IP experiment of mouse lung using 15460-1-AP

    IP result of anti-DLC1 (IP:15460-1-AP, 4ug; Detection:15460-1-AP 1:300) with mouse lung tissue lysate 2400ug.

经过测试的应用

Positive IP detected inmouse lung tissue
Positive IHC detected inhuman spleen tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15460-1-AP targets DLC1 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP,ELISA
Tested Reactivity human, mouse, rat
Immunogen DLC1 fusion protein Ag7732 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deleted in liver cancer 1
Synonyms ARHGAP7, deleted in liver cancer 1, DLC 1, DLC1, HP, HP protein, KIAA1723, p122 RhoGAP, STARD12
Calculated Molecular Weight 171 kDa
GenBank Accession NumberBC054511
Gene Symbol DLC1
Gene ID (NCBI) 10395
RRIDAB_2246077
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for DLC1 antibody 15460-1-APDownload protocol
IF protocol for DLC1 antibody 15460-1-APDownload protocol
IP protocol for DLC1 antibody 15460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols