验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells, HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31304-1-AP targets DIAPH2 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | DIAPH2 fusion protein Ag35280 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | diaphanous homolog 2 (Drosophila) |
| 别名 | DIA, DIA2, DIAPH2, Diaphanous related formin 2, DRF2, FLJ11167, mDia3, POF, POF2, Protein diaphanous homolog 2 |
| 计算分子量 | 126kDa |
| 观测分子量 | 126-130 kDa |
| GenBank蛋白编号 | NM_006729.4 |
| 基因名称 | DIAPH2 |
| Gene ID (NCBI) | 1730 |
| RRID | AB_3669941 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O60879 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In vertebrates, three DRF genes (DIAPH1, DIAPH2 and DIAPH3) are the orthologs of the Drosophila melanogaster diaphanous (dia) gene, which has important roles in fertility and hearing in the fly. DIAPH1 and DIAPH3 have been associated with different types of hearing loss (HL). DIAPH2, located on chromosome Xq21.33, until now it has only been linked to premature ovarian failure 2 (POF2A). (PMID: 36689403)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DIAPH2 antibody 31304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |