验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, MCF-7 cells, SKOV-3 cells, mouse heart tissue, mouse ovary tissue, mouse spleen tissue, rat spleen tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive FC (Intra) detected in | HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14877-1-AP targets DHODH in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, goat |
| 免疫原 |
CatNo: Ag6649 Product name: Recombinant human DHODH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-395 aa of BC065245 Sequence: MAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dihydroorotate dehydrogenase |
| 别名 | PYRD, DHOdehase, dihydroorotate dehydrogenase, Dihydroorotate dehydrogenase (quinone), mitochondrial, Dihydroorotate oxidase |
| 计算分子量 | 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC065245 |
| 基因名称 | DHODH |
| Gene ID (NCBI) | 1723 |
| RRID | AB_2091723 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q02127 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DHODH(Dihydroorotate dehydrogenase) catalyzes the fourth enzymatic step in de novo pyrimidine biosynthesis.DHO dehydrogenase is a monofunctional protein which, in most eukaryotic organisms, is located on the outer surface of the inner mitochondrial membrane.Defects in DHODH are the cause of postaxial acrofacial dysostosis (POADS).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DHODH antibody 14877-1-AP | Download protocol |
| IHC protocol for DHODH antibody 14877-1-AP | Download protocol |
| IP protocol for DHODH antibody 14877-1-AP | Download protocol |
| WB protocol for DHODH antibody 14877-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Nature DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer.
| ||
Science Stimulation of de novo pyrimidine synthesis by growth signaling through mTOR and S6K1. | ||
Cancer Discov PTEN Regulates Glutamine Flux to Pyrimidine Synthesis and Sensitivity to Dihydroorotate Dehydrogenase Inhibition. | ||
Cancer Cell Cancer-selective metabolic vulnerabilities in MYC-amplified medulloblastoma
| ||
Cell Metab Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.
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