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验证数据展示

  • WB analysis using 10191-2-AP (same clone as 10191-2-PBS)

    WB analysis using 10191-2-AP (same clone as 10191-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10191-2-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10191-2-AP (same clone as 10191-2-PBS)

    IP experiment of HEK-293 using 10191-2-AP (same clone as 10191-2-PBS)

    IP result of anti-DFF45/DFFA (IP:10191-2-AP, 3ug; Detection:10191-2-AP 1:1000) with HEK-293 cells lysate 2500ug. This data was developed using the same antibody clone with 10191-2-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 10191-2-AP (same clone as 10191-2-PBS)

    IHC staining of human prostate cancer using 10191-2-AP (same clone as 10191-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10191-2-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 10191-2-AP (same clone as 10191-2-PBS)

    IF Staining of HEK-293 using 10191-2-AP (same clone as 10191-2-PBS)

    Immunofluorescent analysis of HEK-293 cells using 10191-2-AP (DFF45/DFFA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10191-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

DFFA/DFF45 Polyclonal antibody, PBS Only

DFFA/DFF45 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10191-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10191-2-PBS targets DFFA/DFF45 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 DFFA/DFF45 fusion protein Ag0244 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DNA fragmentation factor, 45kDa, alpha polypeptide
别名 DFF45, DFFA, DFF45/DFFA, DFF-45, DFF1
计算分子量 37 kDa
观测分子量 37 kDa, 45 kDa
GenBank蛋白编号BC000037
基因名称 DFFA
Gene ID (NCBI) 1676
RRIDAB_2292937
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00273
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. DFFA appears to be a 45-kDa protein despite the predicated MW of 37 kDa.