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DEPC

DEPC (Diethyl pyrocarbonate) 是一种有效的、不可逆的含有组氨酸残基的 RNase 抑制剂。DEPC 可用碳氧基化修饰组氨酸残基从而导致酶失活，可用于防止RNA 酶降解RNA。DEPC 对兔子的中枢化学敏感性具有抑制作用。DEPC 可以修饰 His，Tyr，Ser 和 Thr 残基。

CAS号

1609-47-8

分子式

C6H10O5

主要靶点

Others

仅限科研使用

Cat No : CM02233

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Synonyms

Diethyl pyrocarbonate

验证数据展示

  • CAS No.: 1609-47-8

    DEPC
    CAS#: 1609-47-8

产品信息

DEPC is a potent and irreversible RNase inhibitor containing histidine residues.DEPC can be used to prevent RNA enzyme degradation by modifying histidine residues with carboxylation resulting in enzyme inactivation. DEPC showed inhibitory effect on the central chemical sensitivity of rabbits. DEPC modified His, Tyr, Ser and Thr residues.

CAS号 1609-47-8
分子式 C6H10O5
主要靶点 Others
主要通路 其他
分子量 162.14
纯度 99.58%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件
别名 Diethyl pyrocarbonate

体内活性

Insulin binding to rat liver plasma membranes is inhibited in a time- and dose-dependent fashion by prior treatment of membranes with the histidine-specific reagent diethyl pyrocarbonate.[1]

溶解度

H2O:30.0 mg/mL (185.0 mM),DMSO:90.0 mg/mL (555.1 mM),Sonification is recommended.

参考文献

1.Pilch PF. Modification of the insulin receptor by diethyl pyrocarbonate: effect on insulin binding and action. Biochemistry. 1982;21(22):5638-5644.
2.Daron HH, et al. Inactivation of dihydrofolate reductase from Lactobacillus casei by diethyl pyrocarbonate. Biochemistry. 1982;21(4):737-741.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
×
=
×
C1   V1   C2   V2