验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, mouse testis tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26297-1-AP targets DDHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | DDHD1 fusion protein Ag23620 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | DDHD domain containing 1 |
别名 | Phosphatidic acid-preferring phospholipase A1 homolog, PA-PLA1, KIAA1705, EC:3.1.1.118, EC:3.1.1.111 |
观测分子量 | 100-110 kDa |
GenBank蛋白编号 | BC030703 |
基因名称 | DDHD1 |
Gene ID (NCBI) | 80821 |
RRID | AB_3085852 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8NEL9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDHD1, also known as KIAA1705, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD1 is highly expressed in the brain and testes. DDHD1 negatively controls the formation of a local phosphatidic acid-rich domain in recycling endosomes that serves as a membrane source for neurite outgrowth (PMID: 32850804).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for DDHD1 antibody 26297-1-AP | Download protocol |
IHC protocol for DDHD1 antibody 26297-1-AP | Download protocol |
IF protocol for DDHD1 antibody 26297-1-AP | Download protocol |
IP protocol for DDHD1 antibody 26297-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |