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验证数据展示

  • WB analysis of HepG2 using 19938-1-AP

    WB analysis of HepG2 using 19938-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 19938-1-AP (DAPK1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 19938-1-AP

    IHC staining of human small intestine using 19938-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19938-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with DAPK1 antibody included
  • IHC staining of human small intestine using 19938-1-AP

    IHC staining of human small intestine using 19938-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 19938-1-AP (DAPK1 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with DAPK1 antibody included

DAPK1 Polyclonal antibody

DAPK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19938-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

DAP kinase 1, DAPK, DAPK1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy DAPK1 Ready-To-Use IHC Kit. DAPK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19938-1-AP targets DAPK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 death-associated protein kinase 1
别名 DAP kinase 1, DAPK, DAPK1
计算分子量 160 kDa
GenBank蛋白编号NM_004938
基因名称 DAPK1
Gene ID (NCBI) 1612
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53355
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DAPK1, also named DAPK, belongs to the protein kinase superfamily; CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK1 is a calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. It catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody recognizes isoform1 of DAPK1.

实验方案

Product Specific Protocols
WB protocol for DAPK1 antibody 19938-1-APDownload protocol
IHC protocol for DAPK1 antibody 19938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

J Tradit Complement Med

Effect of electrode configuration in electroacupuncture on ischemic stroke treatment in rats

Authors - Chung-Hsiang Liu
View Article