验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, A549 cells |
Planning an IHC experiment? We recommend our IHCeasy DACT1 Ready-To-Use IHC Kit. DACT1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
27237-1-AP targets DACT1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, rat |
| 免疫原 | DACT1 fusion protein Ag26096 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dapper, antagonist of beta-catenin, homolog 1 (Xenopus laevis) |
| 别名 | DACT1, DAPPER, Dapper homolog 1, DAPPER1, DPR1, FRODO, HDPR1, HNG3, THYEX3 |
| 计算分子量 | 90 kDa |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | NM_001079520 |
| 基因名称 | DACT1 |
| Gene ID (NCBI) | 51339 |
| RRID | AB_3085940 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NYF0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DACT1 (Dapper/Frodo) is a member of the Dapper family, which includes three genes: DACT1, DACT2 and DACT3. As a tumor suppressor, DACT1 is downregulated in multiple tumor types, such as liver, colon and breast cancer . DACT1 is an important modulator of Wnt signaling, interacting with key components of the various Wnt transduction pathways. For instance, DACT1 binds to disheveled homolog 1 (Dvl1) to induce the degradation of Dvl1 and the subsequent inhibition of the Wnt/β-catenin pathway . (PMID: 34252542, PMID: 29456669)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DACT1 antibody 27237-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Androl Urol Levosimendan in rats decreases acute kidney injury after cardiopulmonary resuscitation by improving mitochondrial dysfunction | ||
Biochem Pharmacol SMURF1 leads to the β-catenin signaling-mediated progression of esophageal squamous carcinoma by losing PATZ1-induced CCNG2 transcription |