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验证数据展示

  • WB analysis of mouse liver using 51157-1-AP

    WB analysis of mouse liver using 51157-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 51157-1-AP (Cyp4a12a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat liver using 51157-1-AP

    IHC staining of rat liver using 51157-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 51157-1-AP

    IHC staining of rat liver using 51157-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 51157-1-AP (Cyp4a12a antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cyp4a12a Polyclonal antibody

Cyp4a12a Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51157-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat, human

应用

WB, IHC, ELISA

别名

Cyp4a12, Cyp4a12a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

51157-1-AP targets Cyp4a12a in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 mouse, rat, human
文献引用反应性rat
免疫原

CatNo: Ag0509

Product name: Recombinant mouse Cyp4a12a protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 248-508 aa of NM_177406

Sequence: CKANSACKLAHDHTDQVIKSRRIQLQDEEELEKLKKKRRLDFLDILLFARMENGKSLSDKDLRAEVDTFMFEGHDTTASGISWIFYALATNPEHQQRCRKEIQSLLGDGTSITWNDLDKMPYTTMCIKEALRIYPPVPSVSRELSSPVTFPDGRSLPKGIHVMLSFYGLHHNPTVWPNPEVFDPSRFAPGSSRHSHSFLPFSGGARNCIGKQFAMNELKVAVALTLLRFELLPDPTRVPIPIPRIVLKSKNGIHLHLKKLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 4, subfamily a, polypeptide 12a
别名 Cyp4a12, Cyp4a12a
观测分子量 58-70 kDa
GenBank蛋白编号NM_177406
基因名称 Cyp4a12a
Gene ID (NCBI) 277753
RRIDAB_2881255
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ91WL5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. Mouse Cyp4a12a oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. For modification, the MW of Cyp4a12a is about 58-70 kDa in western blot detection.

实验方案

Product Specific Protocols
WB protocol for Cyp4a12a antibody 51157-1-APDownload protocol
IHC protocol for Cyp4a12a antibody 51157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Exp Cell Res

Effects of PPARs/20-HETE on the renal impairment under diabetic conditions.

Authors - Shumei Ding
View Article