Cyp4a12a Polyclonal antibody

Cyp4a12a Polyclonal Antibody for WB, IHC, ELISA
Cat No. 51157-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat, human

应用

WB, IHC, ELISA

Cyp4a12, Cyp4a12a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inrat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

51157-1-AP targets Cyp4a12a in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 mouse, rat, human
文献引用反应性rat
免疫原 Cyp4a12a fusion protein Ag0509 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 4, subfamily a, polypeptide 12a
别名 Cyp4a12, Cyp4a12a
观测分子量 58-70 kDa
GenBank蛋白编号NM_177406
基因名称 Cyp4a12a
Gene ID (NCBI) 277753
RRIDAB_2881255
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ91WL5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. Mouse Cyp4a12a oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. For modification, the MW of Cyp4a12a is about 58-70 kDa in western blot detection.

实验方案

Product Specific Protocols
WB protocol for Cyp4a12a antibody 51157-1-APDownload protocol
IHC protocol for Cyp4a12a antibody 51157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Exp Cell Res

Effects of PPARs/20-HETE on the renal impairment under diabetic conditions.

Authors - Shumei Ding
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