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验证数据展示

  • WB analysis using 31183-1-AP (same clone as 31183-1-PBS)

    WB analysis using 31183-1-AP (same clone as 31183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

  • WB analysis using 31183-1-AP (same clone as 31183-1-PBS)

    WB analysis using 31183-1-AP (same clone as 31183-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31183-1-AP (Cullin 5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 31183-1-AP (same clone as 31183-1-PBS)

    IHC staining of mouse brain using 31183-1-AP (same clone as 31183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 31183-1-AP (same clone as 31183-1-PBS)

    IHC staining of mouse brain using 31183-1-AP (same clone as 31183-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31183-1-AP (Cullin 5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31183-1-PBS in a different storage buffer formulation.

Cullin 5 Polyclonal antibody, PBS Only

Cullin 5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31183-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

31183-1-PBS targets Cullin 5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34620

Product name: Recombinant human CUL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC063306

Sequence: MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cullin 5
别名
计算分子量 91 kDa
观测分子量80 kDa, 90 kDa
GenBank蛋白编号BC063306
基因名称 Cullin 5
Gene ID (NCBI) 8065
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ93034
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cullin family proteins are scaffold proteins for the RING finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. In humans, there are eight members of cullins, including CUL1, 2, 3, 4A, 4B, 5, 7, and CUL9 with an evolutionarily conserved cullin homology domain at the C-terminus, responsible for their interaction with the RING protein, assembling 8 cullin-based RING dependent E3 ubiquitin ligases to target thousands of substrates for ubiquitination and degradation (PMID: 32334051). Cullin 5 is the key scaffolding protein of Cullin RING ligase-5 (CRL-5). Cullin 5 plays positive roles in regulating cell growth, proliferation and physiological and other processes in the human body (PMID: 32194910). Cullin 5 has been detected in the form of double bands in the results of Western blot, which might be caused by neddylation (PMID: 36449095; 31294695).

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