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验证数据展示

  • WB analysis of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    WB analysis of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • WB analysis using 14968-1-AP (same clone as 14968-1-PBS)

    WB analysis using 14968-1-AP (same clone as 14968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHGB antibody included
  • IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHGB antibody included
  • FC experiment of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    FC experiment of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (14968-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using Chromogranin B antibody (14968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using 14968-1-AP (Chromogranin B antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Chromogranin B Polyclonal antibody, PBS Only

Chromogranin B Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA
Cat No. 14968-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

别名

CgB, CHGB, SCG1, Chromogranin-B, PE-11

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHGB Ready-To-Use IHC Kit. CHGB primary antibody included.

产品信息

14968-1-PBS targets Chromogranin B in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6833

Product name: Recombinant human CHGB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 328-677 aa of BC000375

Sequence: HSTHYRASEEEPEYGEEIKGYPGVQAPEDLEWERYRGRGSEEYRAPRPQSEESWDEEDKRNYPSLELDKMAHGYGEESEEERGLEPGKGRHHRGRGGEPRAYFMSDTREEKRFLGEGHHRVQENQMDKARRHPQGAWKELDRNYLNYGEEGAPGKWQQQGDLQDTKENREEARFQDKQYSSHHTAEKRKRLGELFNPYYDPLQWKSSHFERRDNMNDNFLEGEEENELTLNEKNFFPEYNYDWWEKKPFSEDVNWGYEKRNLARVPKLDLKRQYDRVAQLDQLLHYRKKSAEFPDFYDSEEPVSTHQEAENEKDRADQTVLTEDEKKELENLAAMDLELQKIAEKFSQRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromogranin B (secretogranin 1)
别名 CgB, CHGB, SCG1, Chromogranin-B, PE-11
计算分子量 78 kDa
观测分子量 70-100 kDa
GenBank蛋白编号BC000375
基因名称 Chromogranin B
Gene ID (NCBI) 1114
RRIDAB_2081138
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05060
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa. It has been shown that CHGB has a clear degradation band at ~50 kDa (https://www.biorxiv.org/content/10.1101/2019.12.28.890053v1.full.pdf).

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