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验证数据展示

  • WB analysis using 18054-1-AP (same clone as 18054-1-PBS)

    WB analysis using 18054-1-AP (same clone as 18054-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18054-1-AP (CYP7A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 18054-1-AP (same clone as 18054-1-PBS)

    IF Staining of HepG2 using 18054-1-AP (same clone as 18054-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7A1 antibody (18054-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

CYP7A1 Polyclonal antibody, PBS Only

CYP7A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18054-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

18054-1-PBS targets CYP7A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12572

Product name: Recombinant human CYP7A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 154-504 aa of BC101777

Sequence: RPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 7, subfamily A, polypeptide 1
别名 Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase
计算分子量 504 aa, 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC101777
基因名称 CYP7A1
Gene ID (NCBI) 1581
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22680
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.

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