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验证数据展示

  • WB analysis of PC-3 using 10108-2-AP (same clone as 10108-2-PBS)

    WB analysis of PC-3 using 10108-2-AP (same clone as 10108-2-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10108-2-AP (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 10108-2-AP (same clone as 10108-2-PBS)

    IHC staining of human small intestine using 10108-2-AP (same clone as 10108-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 10x lens).. This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CX3CL1 antibody included
  • IHC staining of human small intestine using 10108-2-AP (same clone as 10108-2-PBS)

    IHC staining of human small intestine using 10108-2-AP (same clone as 10108-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 40x lens).. This data was developed using the same antibody clone with 10108-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CX3CL1 antibody included

CX3CL1 Polyclonal antibody, PBS Only

CX3CL1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10108-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, C3Xkine, C X3 C motif chemokine 1, ABCD 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CX3CL1 Ready-To-Use IHC Kit. CX3CL1 primary antibody included.

产品信息

10108-2-PBS targets CX3CL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0161

Product name: Recombinant human CX3CL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 90-245 aa of BC001163

Sequence: DRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X3-C motif) ligand 1
别名 C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, C3Xkine, C X3 C motif chemokine 1, ABCD 3
计算分子量 42 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC001163
基因名称 CX3CL1
Gene ID (NCBI) 6376
RRIDAB_2087154
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78423
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. The human CX3C chemokine carries the chemokine domain on top of an extended mucin-like stalk. CX3CL1 is synthesized as a 50-75 kDa precursor and undergoes glycosylation to yield two mature forms: a 100 kDa cell membrane bound form and an 85 kDa soluble form which is released from the cell surface. The soluble CX3C chemokine has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes.