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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 13385-1-AP (same clone as 13385-1-PBS)

    WB analysis using 13385-1-AP (same clone as 13385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

    IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

    IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • IF Staining of COLO 320 using 13385-1-AP (same clone as 13385-1-PBS)

    IF Staining of COLO 320 using 13385-1-AP (same clone as 13385-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CPN1 antibody (13385-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13385-1-AP (same clone as 13385-1-PBS)

    IF Staining of HepG2 using 13385-1-AP (same clone as 13385-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CPN1 antibody (13385-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

CPN1 Polyclonal antibody, PBS Only

CPN1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13385-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13385-1-PBS targets CPN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CPN1 fusion protein Ag4035 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carboxypeptidase N, polypeptide 1
别名
计算分子量 458 aa, 52 kDa
观测分子量 40~50 kDa
GenBank蛋白编号BC027897
基因名称 CPN1
Gene ID (NCBI) 1369
RRIDAB_2292159
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15169
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.