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验证数据展示

  • WB analysis using 13274-1-AP (same clone as 13274-1-PBS)

    WB analysis using 13274-1-AP (same clone as 13274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13274-1-AP (CPEB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)

    IHC staining of human liver cancer using 13274-1-AP (same clone as 13274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13274-1-AP (CPEB1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13274-1-AP (same clone as 13274-1-PBS)

    IF Staining of HeLa using 13274-1-AP (same clone as 13274-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CPEB1 antibody (13274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13274-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CPEB1 Polyclonal antibody, PBS Only

CPEB1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13274-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13274-1-PBS targets CPEB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3994

Product name: Recombinant human CPEB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 188-486 aa of BC035348

Sequence: WDLLEAPKDPFSIEREARLHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPRCPPKGYVYLVFELEKSVRSLLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQRLDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTTKFTKKVQIDPYLEDSLCHICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQKNRDSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytoplasmic polyadenylation element binding protein 1
别名
计算分子量 486 aa, 54 kDa
观测分子量 60-70kDa
GenBank蛋白编号BC035348
基因名称 CPEB1
Gene ID (NCBI) 64506
RRIDAB_2083771
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZB8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling. This is a rabbit polyclonal antibody raised against residues near the C terminus of human CPEB1.

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