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验证数据展示

  • WB analysis of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

    WB analysis of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

    WB result of COX5B antibody (11418-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX5B transfected HeLa cells. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • WB analysis using 11418-2-AP (same clone as 11418-2-PBS)

    WB analysis using 11418-2-AP (same clone as 11418-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11418-2-AP (COX5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 11418-2-AP (same clone as 11418-2-PBS)

    IHC staining of human liver cancer using 11418-2-AP (same clone as 11418-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11418-2-AP (COX5B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

    IF Staining of HeLa using 11418-2-AP (same clone as 11418-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX5B antibody (11418-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11418-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

COX5B Polyclonal antibody, PBS Only

COX5B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11418-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

COXVB

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

11418-2-PBS targets COX5B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1986

Product name: Recombinant human COX5B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-129 aa of BC006229

Sequence: MASRLLRGAGTLAAQALRARGPSGAAAMRSMASGGGVPTDEEQATGLEREIMLAAKKGLDPYNVLAPKGASGTREDPNLVPSISNKRIVGCICEEDNTSVVWFWLHKGEAQRCPRCGAHYKLVPQQLAH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome c oxidase subunit Vb
别名 COXVB
计算分子量 129 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC006229
基因名称 COX5B
Gene ID (NCBI) 1329
RRIDAB_2245376
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10606
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COX5B belongs to the cytochrome c oxidase subunit 5B family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX5B and other 3 genes CKB, HBB, MBP are involved in respiration.(PMID:21295140)

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