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验证数据展示

  • WB analysis using 31425-1-AP

    WB analysis using 31425-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31425-1-AP (CORO7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 31425-1-AP

    IP experiment of HeLa using 31425-1-AP

    IP result of anti-CORO7 (IP:31425-1-AP, 4ug; Detection:31425-1-AP 1:2000) with HeLa cells lysate 980 ug.

  • IHC staining of human ovary cancer using 31425-1-AP

    IHC staining of human ovary cancer using 31425-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31425-1-AP (CORO7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CORO7 Polyclonal antibody

CORO7 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 31425-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

CRN7, Coronin-7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, MDA-MB-231 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31425-1-AP targets CORO7 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原 CORO7 fusion protein Ag35016 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coronin 7
别名 CRN7, Coronin-7
计算分子量101 kDa
观测分子量 100 kDa
GenBank蛋白编号NM_024535
基因名称 CORO7
Gene ID (NCBI) 79585
RRIDAB_3669976
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP57737
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CORO7 also known as CRN7 belongs to the WD repeat coronin family, and functions as an F-actin regulator directing anterograde Golgi to endosome transport(PMID: 15327992).

实验方案

Product Specific Protocols
WB protocol for CORO7 antibody 31425-1-APDownload protocol
IHC protocol for CORO7 antibody 31425-1-APDownload protocol
IP protocol for CORO7 antibody 31425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols