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验证数据展示

  • WB analysis of mouse heart using 12481-1-AP (same clone as 12481-1-PBS)

    WB analysis of mouse heart using 12481-1-AP (same clone as 12481-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12481-1-AP (COQ6 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

  • WB analysis using 12481-1-AP (same clone as 12481-1-PBS)

    WB analysis using 12481-1-AP (same clone as 12481-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12481-1-AP (COQ6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 12481-1-AP (same clone as 12481-1-PBS)

    IHC staining of mouse ovary using 12481-1-AP (same clone as 12481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12481-1-AP (COQ6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse ovary using 12481-1-AP (same clone as 12481-1-PBS)

    IHC staining of mouse ovary using 12481-1-AP (same clone as 12481-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12481-1-AP (COQ6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12481-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

COQ6 Polyclonal antibody, PBS Only

COQ6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12481-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12481-1-PBS targets COQ6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3164

Product name: Recombinant human COQ6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 151-468 aa of BC014181

Sequence: ALTKQLEAVSDRVTVLYRSKAIRYTWPCPFPMADSSPWVHITLGDGSTFQTKLLIGADGHNSGVRQAVGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFVDAVNSAFWSDADHTDFIDTAGAMLQYAVSLLKPTKVSARQLPPSVARVDAKSRVLFPLGLGHAAEYVRPRVALIGDAAHRVHPLAGQGVNMGFGDISSLAHHLSTAAFNGKDLGSVSHLTGYETERQRHNTALLAATDLLKRLYSTSASPLVLLRTWGLQATNAVSPLKEQIMAFASK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coenzyme Q6 homolog, monooxygenase (S. cerevisiae)
别名
计算分子量 468 aa, 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC014181
基因名称 COQ6
Gene ID (NCBI) 51004
RRIDAB_10644143
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2Z9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COQ6(Coenzyme Q10 monooxygenase 6) is also named CGI-10 and belongs to the ubiH/COQ6 family. It is an evolutionarily conserved flavin-dependent monooxygenase required for the biosynthesis of coenzyme Q10 or ubiquinone. It has 3 isoforms with a molecular mass of 51 kDa, 43 kDa and 49 kDa. In the inner ear, Coq6 is expressed in the spiral ganglion, stria vascularis cells, and spiral ligament(PMID:21540551 ). Defects in COQ6 are the cause of coenzyme Q10 deficiency, primary, type 6 (COQ10D6).