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验证数据展示

  • WB analysis using 31539-1-AP

    WB analysis using 31539-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31539-1-AP (COG4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 31539-1-AP

    WB analysis using 31539-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31539-1-AP (COG4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 31539-1-AP

    IHC staining of mouse testis using 31539-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31539-1-AP (COG4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COG4 Polyclonal antibody

COG4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31539-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

COD1, COG complex subunit 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, mouse spermatophore tissue, MRC-5 cells, hTERT-RPE1 cells, mouse testis tisuue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31539-1-AP targets COG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 COG4 fusion protein Ag35295 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 component of oligomeric golgi complex 4
别名 COD1, COG complex subunit 4
计算分子量89kDa，785aa
观测分子量89 kDa
GenBank蛋白编号NM_001365426.1
基因名称 COG4
Gene ID (NCBI) 25839
RRIDAB_3670028
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H9E3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COG complex subunit 4 is present in high molecular weight complexes that govern membrane movement between the Golgi and ER. Cog4 dependent trafficking and secretion of ECM components are required for protrusion and extension of the epithelial projections of the inner ear (PMID: 30287385). Loss-of-function COG4-mutant cells have impaired glycosylation and reduced COG4 protein levels (PMID: 30290151).

实验方案

Product Specific Protocols
WB protocol for COG4 antibody 31539-1-APDownload protocol
IHC protocol for COG4 antibody 31539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols