验证数据展示
经过测试的应用
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67089-1-Ig targets CNTN2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag28301 Product name: Recombinant human CNTN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 865-943 aa of NM_005076 Sequence: MRTAGLDTSARVSGLHPNTKYHVTVRAYNRAGTGPASPSANATTMKPPPRRPPGNISWTFSSSSLSIKWDPVVPFRNESA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | contactin 2 (axonal) |
| 别名 | 1D3G5, Axonin 1, AXT, Contactin2, Contactin-2 |
| 计算分子量 | 113 kDa |
| 观测分子量 | 113-135 kDa |
| GenBank蛋白编号 | NM_005076 |
| 基因名称 | CNTN2 |
| Gene ID (NCBI) | 6900 |
| RRID | AB_2918481 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q02246 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Contactin-2 (CNTN2), also known as CNTN2 or axonal glycoprotein TAG-1, is a cell adhesion molecule anchored within the cell membrane that is a member of the immunoglobulin family. It is reported to be transiently expressed in specific neurons during the fetal period (PMID: 27621318, 29777776). CNTN2 consists of six Ig domains followed by four fibronectin domains, and it is tethered to the cell surface by a glycosylphosphatidylinositol anchor. CNTN2 levels may reflect impairment of brain development and indirectly brain iron stores, which are likely deficient in infants of maternal IDA or chronic hypoxic states like IDMs (PMID: 26718491).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CNTN2 antibody 67089-1-Ig | Download protocol |
| IHC protocol for CNTN2 antibody 67089-1-Ig | Download protocol |
| WB protocol for CNTN2 antibody 67089-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
