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验证数据展示

  • WB analysis using 10796-1-AP (same clone as 10796-1-PBS)

    WB analysis using 10796-1-AP (same clone as 10796-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10796-1-AP (CNTFR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 10796-1-AP (same clone as 10796-1-PBS)

    IHC staining of mouse brain using 10796-1-AP (same clone as 10796-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 10796-1-AP (same clone as 10796-1-PBS)

    IHC staining of mouse brain using 10796-1-AP (same clone as 10796-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10796-1-AP (CNTFR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10796-1-PBS in a different storage buffer formulation.

CNTFR Polyclonal antibody, PBS Only

CNTFR Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10796-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10796-1-PBS targets CNTFR in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1189

Product name: Recombinant human CNTFR protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-372 aa of BC001492

Sequence: MAAPVPWACCAVLAAAAAVVYAQRHSPQEAPHVQYERLGSDVTLPCGTANWDAAVTWRVNGTDLAPDLLNGSQLVLHGLELGHSGLYACFHRDSWHLRHQVLLHVGLPPREPVLSCRSNTYPKGFYCSWHLPTPTYIPNTFNVTVLHGSKIMVCEKDPALKNRCHIRYMHLFSTIKYKVSISVSNALGHNATAITFDEFTIVKPDPPENVVARPVPSNPRRLEVTWQTPSTWPDPESFPLKFFLRYRPLILDQWQHVELSDGTAHTITDAYAGKEYIIQVAAKDNEIGTWSDWSVAAHATPWTEEPRHLTTEAQAAETTTSTTSSLAPPPTTKICDPGELGSGGGPSAPFLVSVPITLALAAAAATASSLLI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ciliary neurotrophic factor receptor
别名
计算分子量 41 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC001492
基因名称 CNTFR
Gene ID (NCBI) 1271
RRIDAB_2083194
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP26992
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ciliary neurotrophic factor (CNTF) is a pluripotent neurocytokine. The functional receptor complex of CNTF is composed of the CNTF receptor alpha (CNTFR), gp130 and the leukemia inhibitory factor receptor (LIFR) (PMID: 11943154). Binding of CNTF to CNTFR recruits gp130 and LIFR, which leads to activation of JAK/STAT and Ras/Raf/MAPK pathways.

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