验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
26027-1-AP targets CHST2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag23293 Product name: Recombinant human CHST2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 338-530 aa of BC105010 Sequence: ASSRIRSRHGLIRESLQVVRSRDPRAHRMPFLEAAGHKLGAKKEGVGGPADYHALGAMEVICNSMAKTLQTALQPPDWLQGHYLVVRYEDLVGDPVKTLRRVYDFVGLLVSPEMEQFALNMTSGSGSSSKPFVVSARNATQAANAWRTALTFQQIKQVEEFCYQPMAVLGYERVNSPEEVKDLSKTLLRKPRL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 |
| 别名 | C6ST, CHST2, GlcNAc6ST 1, GN6ST, Gn6ST 1, GST 2, GST2 |
| 观测分子量 | 50-52 kDa, 58-60 kDa |
| GenBank蛋白编号 | BC105010 |
| 基因名称 | CHST2 |
| Gene ID (NCBI) | 9435 |
| RRID | AB_2880342 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y4C5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CHST2 encodes N-Acetylglucosamine-6-sulfotransferase-1 (GlcNAc6ST-1), a Golgi-resident glycoprotein that is responsible for sulfation of the L-selectin ligand on endothelial cells. Two isoforms of CHST2 exist due to the alternative splicing. This antibody recognizes both short form and long form, which migrates around 50-52 kDa and 58-60 kDa, respectively.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CHST2 antibody 26027-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |