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验证数据展示

  • WB analysis using 19424-1-AP (same clone as 19424-1-PBS)

    WB analysis using 19424-1-AP (same clone as 19424-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 19424-1-AP (same clone as 19424-1-PBS)

    WB analysis of HEK-293 using 19424-1-AP (same clone as 19424-1-PBS)

    WB result of CHCHD2 antibody (19424-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 19424-1-AP (same clone as 19424-1-PBS)

    IHC staining of human lung cancer using 19424-1-AP (same clone as 19424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHCHD2 antibody included
  • IHC staining of human lung cancer using 19424-1-AP (same clone as 19424-1-PBS)

    IHC staining of human lung cancer using 19424-1-AP (same clone as 19424-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHCHD2 antibody included
  • IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    IP experiment of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

    IP result of anti-CHCHD2 (IP:19424-1-AP, 4ug; Detection:19424-1-AP 1:1000) with HepG2 cells lysate 2520 ug. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CHCHD2 Polyclonal antibody, PBS Only

CHCHD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 19424-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CHCHD2 Ready-To-Use IHC Kit. CHCHD2 primary antibody included.

产品信息

19424-1-PBS targets CHCHD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13752

Product name: Recombinant human CHCHD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-151 aa of BC003079

Sequence: MPRGSRSRTSRMAPPASRAPQMRAAPRPAPVAQPPAAAPPSAVGSSAAAPRQPGLMAQMATTAAGVAVGSAVGHTLGHAITGGFSGGSNAEPARPDITYQEPQGTQPAQQQQPCLYEIKQFLECAQNQGDIKLCEGFNEVLKQCRLANGLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil-helix-coiled-coil-helix domain containing 2
别名
计算分子量 151 aa, 16 kDa
观测分子量 16-18 kDa
GenBank蛋白编号BC003079
基因名称 CHCHD2
Gene ID (NCBI) 51142
RRIDAB_10638907
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6H1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

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