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验证数据展示

  • WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

    WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60312-1-Ig (CDK2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK2 antibody included
  • IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK2 antibody included
  • FC experiment of HepG2 using 60312-1-Ig (same clone as 60312-1-PBS)

    FC experiment of HepG2 using 60312-1-Ig (same clone as 60312-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CDK2 Monoclonal antibody (60312-1-Ig, Clone:4E2E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

    IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (60312-1-Ig, Clone: 4E2E10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

CDK2 Monoclonal antibody, PBS Only

CDK2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 60312-1-PBS

产品说明书

CloneNo. 4E2E10

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

4E2E10, CDKN2, Cell division protein kinase 2, Cyclin dependent kinase 2, Cyclin-dependent kinase 2

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CDK2 Ready-To-Use IHC Kit. CDK2 primary antibody included.

产品信息

60312-1-PBS targets CDK2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21310

Product name: Recombinant human CDK2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 8-210 aa of BC003065

Sequence: EKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEID

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase 2
别名 4E2E10, CDKN2, Cell division protein kinase 2, Cyclin dependent kinase 2, Cyclin-dependent kinase 2
计算分子量 33 kDa
观测分子量 30 kDa
GenBank蛋白编号BC003065
基因名称 CDK2
Gene ID (NCBI) 1017
RRIDAB_2881424
偶联类型 Unconjugated
形式Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP24941
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

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