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Anti-Human CD80 (B7-1) (2D10.4)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

2D10.4

Cat No : 65083-1-Ig

Print datasheet

Synonyms

Activation B7 1 antigen, B7, B7 1, BB1, CD28LG, CD28LG1, CD80, CD80 (B7-1), CD80 molecule, CTLA 4 counter receptor B7.1, LAB7

验证数据展示

  • FC experiment of Daudi using 65083-1-Ig

    FC experiment of Daudi using 65083-1-Ig

    1X10^6 Daudi cells were surface stained with 0.25 ug Anti-Human CD80 (B7-1) (65083-1-Ig, Clone: 2D10.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

经过测试的应用

Positive FC detected inDaudi cells
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65083-1-Ig targets CD80 (B7-1) in FC applications and shows reactivity with Human samples.

Tested Applications FC
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD80 molecule
Synonyms Activation B7 1 antigen, B7, B7 1, BB1, CD28LG, CD28LG1, CD80, CD80 (B7-1), CD80 molecule, CTLA 4 counter receptor B7.1, LAB7
Calculated Molecular Weight 33 kDa
GenBank Accession NumberBC042665
Gene Symbol CD80
Gene ID (NCBI) 941
RRIDAB_2918386
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

实验方案

Product Specific Protocols
FC protocol for CD80 (B7-1) antibody 65083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols