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验证数据展示

  • WB analysis using 32152-1-AP

    WB analysis using 32152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32152-1-AP (CD79B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 32152-1-AP

    IHC staining of mouse spleen using 32152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32152-1-AP (CD79b antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 32152-1-AP

    IHC staining of mouse spleen using 32152-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32152-1-AP (CD79b antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD79b Polyclonal antibody

CD79b Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32152-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Igb, Ig-beta, Immunoglobulin-associated B29 protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32152-1-AP targets CD79b in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2113

Product name: Recombinant Mouse CD79B protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 26-158 aa of NM_008339.3

Sequence: VPAMTSSDLPLNFQGSPCSQIWQHPRFAAKKRSSMVKFHCYTNHSGALTWFRKRGSQQPQELVSEEGRIVQTQNGSVYTLTIQNIQYEDNGIYFCKQKCDSANHNVTDSCGTELLVLGFSTLDQLKRRNTLKD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD79B antigen
别名 B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Igb, Ig-beta, Immunoglobulin-associated B29 protein
计算分子量26 kDa
观测分子量35 kDa
GenBank蛋白编号NM_008339.3
基因名称 Cd79b
Gene ID (NCBI) 15985
RRIDAB_3670210
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP15530
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).

实验方案

Product Specific Protocols
IHC protocol for CD79b antibody 32152-1-APDownload protocol
WB protocol for CD79b antibody 32152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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