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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using 65157-1-Ig

    FC experiment of human peripheral blood lymphocytes using 65157-1-Ig

    1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD72 (65157-1-Ig, Clone: 3F3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

Anti-Human CD72 (3F3)

CD72 Monoclonal Antibody for FC
Cat No. 65157-1-Ig

产品说明书

CloneNo. 3F3

宿主/亚型

Mouse / IgG2b, kappa

种属反应性

Human

应用

FC

别名

CD72, CD72 molecule, CD72b, Lyb 2, LYB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65157-1-Ig targets CD72 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 Normal human lymphocytes from a lymph node 种属同源性预测
宿主/亚型 Mouse / IgG2b, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD72 molecule
别名 CD72, CD72 molecule, CD72b, Lyb 2, LYB2
GenBank蛋白编号BC011734
基因名称 CD72
Gene ID (NCBI) 971
RRIDAB_2918439
偶联类型 Unconjugated
形式 Liquid
纯化方式Purified by precipitation and chromatography
UNIPROT IDP21854
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).

实验方案

Product Specific Protocols
FC protocol for CD72 antibody 65157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols