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验证数据展示

  • WB analysis using 31243-1-AP (same clone as 31243-1-PBS)

    WB analysis using 31243-1-AP (same clone as 31243-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31243-1-AP (CD45/PTPRC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 31243-1-AP (same clone as 31243-1-PBS)

    IHC staining of mouse spleen using 31243-1-AP (same clone as 31243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31243-1-AP (CD45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 31243-1-AP (same clone as 31243-1-PBS)

    IHC staining of mouse spleen using 31243-1-AP (same clone as 31243-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31243-1-AP (CD45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 31243-1-AP (same clone as 31243-1-PBS)

    IF Staining of RAW 264.7 using 31243-1-AP (same clone as 31243-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD45 antibody (31243-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31243-1-PBS in a different storage buffer formulation.

Mouse CD45 Polyclonal antibody, PBS Only

CD45 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31243-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Ptprc, EC:3.1.3.48, LCA, L-CA, Leukocyte common antigen

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

31243-1-PBS targets CD45 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg0845

Product name: Recombinant Mouse CD45 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 26-566 aa of NM_001111316.2

Sequence: QTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 Ptprc, EC:3.1.3.48, LCA, L-CA, Leukocyte common antigen
计算分子量145 kDa
观测分子量180 kDa
GenBank蛋白编号NM_001111316.2
基因名称 CD45
Gene ID (NCBI) 19264
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06800-4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

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