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验证数据展示

  • WB analysis using 31981-1-AP

    WB analysis using 31981-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31981-1-AP (CD44 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of rat spleen using 31981-1-AP

    IF Staining of rat spleen using 31981-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD44 antibody (31981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat spleen using 31981-1-AP

    IF Staining of rat spleen using 31981-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD44 antibody (31981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD44 Polyclonal antibody

CD44 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 31981-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF-P, ELISA

别名

CD44 antigen, ECMR-III, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, Hermes antigen

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat lung tissue, rat spleen tissue
Positive IF-P detected inrat spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31981-1-AP targets CD44 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg1458

Product name: Recombinant Rat CD44 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 22-271 aa of XM_006234631.3

Sequence: QQQIDLNITCRYAGVFHVEKNGRYSISRTEAADLCEAFNTTLPTMAQMELALSKGFETCRYGFIEGHVVIPRIHPNAICAANNTGVYILLASNTSHYDTYCFNASAPLEEDCTSVTDLPNSFDGPVTITIVNRDGTRYSKKGEYRTHQEDIDASNIIDEDVSSGSTIEKSTPEGYILHTDLPTSQPTGDRDDAFFIGSTLATSDGDSSMDPRGGFDTVTHGSELAGHSSGNQDSGVTTTSGPARRPQIPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cd44 molecule
别名 CD44 antigen, ECMR-III, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, Hermes antigen
计算分子量40 kDa
观测分子量 80-95 kDa
GenBank蛋白编号XM_006234631.3
基因名称 Cd44
Gene ID (NCBI) 25406
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO70509
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
IF protocol for CD44 antibody 31981-1-APDownload protocol
WB protocol for CD44 antibody 31981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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