验证数据展示
经过测试的应用
| Positive WB detected in | J774A.1 cells, RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31446-1-AP targets CD40 in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD40 antigen |
| 别名 | Tnfrsf5, CD40 antigen, Bp50 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | NM_011611.2 |
| 基因名称 | Cd40 |
| Gene ID (NCBI) | 21939 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P27512-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD40, also known as TNFRSF5 or Bp50, is a member of the tumor necrosis factor (TNF) receptor (TNFR) family. It is expressed on a wide range of cell types including B cells, macrophages, and dendritic cells (PMID: 19426221). CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD40 antibody 31446-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |