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CD3 Polyclonal antibody

CD3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse, rat

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17617-1-AP

Print datasheet

Synonyms

CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE

验证数据展示

  • WB analysis using 17617-1-AP

    WB analysis using 17617-1-AP

    Jurkat cells (CD3+), MOLT-4 cells (CD3+), U-937 cells (CD3-), Raji cells (CD3-), mouse thymus tissue, and rat thymus tissue were subjected to SDS PAGE followed by western blot with 17617-1-AP (CD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of human tonsillitis using 17617-1-AP

    IF Staining of human tonsillitis using 17617-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

  • IF Staining of human tonsillitis using 17617-1-AP

    IF Staining of human tonsillitis using 17617-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

  • IHC staining of human tonsillitis using 17617-1-AP

    IHC staining of human tonsillitis using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • IHC staining of human tonsillitis using 17617-1-AP

    IHC staining of human tonsillitis using 17617-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD3 antibody included
  • FC experiment of Jurkat using 17617-1-AP

    FC experiment of Jurkat using 17617-1-AP

    1X10^6 Jurkat cells were stained with 0.5ug CD3 antibody (17617-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • IP experiment of Jurkat using 17617-1-AP

    IP experiment of Jurkat using 17617-1-AP

    IP Result of anti-CD3 (IP:17617-1-AP, 4ug; Detection:17617-1-AP 1:1000) with Jurkat cells lysate 3600ug.

经过测试的应用

Positive WB detected inJurkat cells, human spleen tissue, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human lymphoma tissue, human pancreas cancer tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

17617-1-AP targets CD3 in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications Cell treatment, FC, IF, IHC, WB
Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen CD3 fusion protein Ag11797 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE
Calculated molecular weight 207 aa, 23 kDa
Observed molecular weight 23-25 kDa
GenBank accession numberBC049847
Gene symbol CD3E
UniprotP07766
Gene ID (NCBI) 916
RRIDAB_1939430
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.

实验方案

Product Specific Protocols
WB protocol for CD3 antibody 17617-1-APDownload protocol
IHC protocol for CD3 antibody 17617-1-APDownload protocol
IF protocol for CD3 antibody 17617-1-APDownload protocol
IP protocol for CD3 antibody 17617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Nat Commun

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Authors - Fan Zou
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humanIHC

Cancer Res

M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma.

Authors - Xinyao Qiu
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mouseIHC

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
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mouseFC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
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humanIHC

Cell Mol Immunol

The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/β-catenin signaling pathway.

Authors - Rilin Deng
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humanIHC,FC

Clin Transl Med

c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis.

Authors - Lan Zhang
View Article