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- KD/KO Validated
CD3 Polyclonal antibody
CD3 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, IP, ELISA and More (2)
Conjugate
Unconjugated
125
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue, human lymphoma tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human pancreas cancer tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue, mouse spleen tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17617-1-AP targets CD3 in WB, IHC, IF-P, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, Cell treatment |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | CD3 fusion protein Ag11797 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
Synonyms | CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E |
Calculated Molecular Weight | 207 aa, 23 kDa |
Observed Molecular Weight | 23-25 kDa |
GenBank Accession Number | BC049847 |
Gene Symbol | CD3 |
Gene ID (NCBI) | 916 |
ENSEMBL Gene ID | ENSG00000198851 |
RRID | AB_1939430 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P07766 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD3 antibody 17617-1-AP | Download protocol |
IHC protocol for CD3 antibody 17617-1-AP | Download protocol |
IF protocol for CD3 antibody 17617-1-AP | Download protocol |
IP protocol for CD3 antibody 17617-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Blood C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse | ||
Nat Commun Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56. | ||
J Adv Res Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model | ||
Cancer Res M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma. | ||
Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A |