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CD3 Polyclonal antibody

CD3 Polyclonal Antibody for WB, IP, IF-P, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse, rat

Applications

WB, IP, IF-P, FC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17617-1-AP

Print datasheet

Synonyms

CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE



经过测试的应用

Positive WB detected inJurkat cells, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue, mouse spleen tissue, human lymphoma tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human pancreas cancer tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, mouse spleen tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17617-1-AP targets CD3 in WB, IP, IF, FC, IHC, ELISA, Cell treatment applications and shows reactivity with human, pig, mouse, rat samples.

Tested Applications WB, IP, IF-P, FC, IHC, ELISA Application Description
Cited ApplicationsWB, IF, FC, IHC, Cell treatment
Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen CD3 fusion protein Ag11797 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE
Calculated Molecular Weight 207 aa, 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3E
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
RRIDAB_1939430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.

实验方案

Product Specific Protocols
WB protocol for CD3 antibody 17617-1-APDownload protocol
IHC protocol for CD3 antibody 17617-1-APDownload protocol
IF protocol for CD3 antibody 17617-1-APDownload protocol
IP protocol for CD3 antibody 17617-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Blood

C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse

Authors - Li-Xue Yang
humanIHC

Nat Commun

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Authors - Fan Zou
ratIHC

J Adv Res

Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model

Authors - Haonan Ruan
humanIHC

Cancer Res

M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma.

Authors - Xinyao Qiu
mouseIHC

Br J Pharmacol

Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A

Authors - Lu Zhang
mouseFC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang