CD3 Polyclonal antibody
CD3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, pig, mouse, rat
Applications
FC, IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, human spleen tissue, pig spleen tissue, MOLT-4 cells, mouse thymus tissue, rat thymus tissue |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue, human lung cancer tissue, human breast cancer tissue, mouse colon tissue, human lymphoma tissue, human pancreas cancer tissue, rat thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue |
Positive FC detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
17617-1-AP targets CD3 in WB, IP, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | Cell treatment, FC, IF, IHC, WB |
Tested Reactivity | human, pig, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | CD3 fusion protein Ag11797 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD3e molecule, epsilon (CD3-TCR complex) |
Synonyms | CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE |
Calculated molecular weight | 207 aa, 23 kDa |
Observed molecular weight | 23-25 kDa |
GenBank accession number | BC049847 |
Gene symbol | CD3E |
Uniprot | P07766 |
Gene ID (NCBI) | 916 |
RRID | AB_1939430 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.
实验方案
Product Specific Protocols | |
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WB protocol for CD3 antibody 17617-1-AP | Download protocol |
IHC protocol for CD3 antibody 17617-1-AP | Download protocol |
IF protocol for CD3 antibody 17617-1-AP | Download protocol |
IP protocol for CD3 antibody 17617-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56. | ||
Cancer Res M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma. | ||
Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A | ||
Bioact Mater Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect. | ||
Cell Mol Immunol The innate immune effector ISG12a promotes cancer immunity by suppressing the canonical Wnt/β-catenin signaling pathway. | ||
Clin Transl Med c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis. |