CD3 Delta Polyclonal antibody

CD3 Delta Polyclonal Antibody for FC, IF-P, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat


FC, IF-P, IP, WB, ELISA and More (1)



Cat No : 16669-1-AP

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CD3 delta, CD3D, CD3-delta, T cell receptor T3 delta chain, T3D, TCRδ


Positive WB detected inJurkat cells, mouse thymus tissue, rat thymus tissue, MOLT-4 cells
Positive IP detected inJurkat cells
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inJurkat cells


Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


16669-1-AP targets CD3 Delta in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF-P, IP, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CD3 Delta fusion protein Ag10148 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD3d molecule, delta (CD3-TCR complex)
Synonyms CD3 delta, CD3D, CD3-delta, T cell receptor T3 delta chain, T3D, TCRδ
Calculated Molecular Weight 171 aa, 19 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC070321
Gene Symbol CD3D
Gene ID (NCBI) 915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.


Product Specific Protocols
WB protocol for CD3 Delta antibody 16669-1-APDownload protocol
IF protocol for CD3 Delta antibody 16669-1-APDownload protocol
IP protocol for CD3 Delta antibody 16669-1-APDownload protocol
Standard Protocols
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