CD3 Delta Polyclonal antibody

CD3 Delta Polyclonal Antibody for FC, IF-P, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF-P, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16669-1-AP

Print datasheet

Synonyms

CD3 delta, CD3D, CD3-delta, T cell receptor T3 delta chain, T3D, TCRδ



经过测试的应用

Positive WB detected inJurkat cells, mouse thymus tissue, rat thymus tissue, MOLT-4 cells
Positive IP detected inJurkat cells
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16669-1-AP targets CD3 Delta in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF-P, IP, WB, ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CD3 Delta fusion protein Ag10148 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD3d molecule, delta (CD3-TCR complex)
Synonyms CD3 delta, CD3D, CD3-delta, T cell receptor T3 delta chain, T3D, TCRδ
Calculated Molecular Weight 171 aa, 19 kDa
Observed Molecular Weight 20-25 kDa
GenBank Accession NumberBC070321
Gene Symbol CD3D
Gene ID (NCBI) 915
RRIDAB_10598004
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04234
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

实验方案

Product Specific Protocols
WB protocol for CD3 Delta antibody 16669-1-APDownload protocol
IF protocol for CD3 Delta antibody 16669-1-APDownload protocol
IP protocol for CD3 Delta antibody 16669-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cancer Res

Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.

Authors - Binghao Li

Clin Cancer Res

Changes in Expression of Genes Representing Key Biologic Processes after Neoadjuvant Chemotherapy in Breast Cancer, and Prognostic Implications in Residual Disease.

Authors - Marie Klintman
mouseIF

Inflamm Regen

Testosterone upregulates glial cell line-derived neurotrophic factor (GDNF) and promotes neuroinflammation to enhance glioma cell survival and proliferation

Authors - Kouminin Kanwore
humanIHC

Ann Diagn Pathol

Diagnostic utility of the aberrant immunohistochemical expression of CD3 molecules for peripheral T-cell lymphomas.

Authors - Tsugumi Satoh
mouseWB,IP

Cell Rep

cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T cells

Authors - Yuji Fukushima
mouseIF

Cell Discov

Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure

Authors - Jie Yin