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验证数据展示

  • ELISA experiment of Mouse PD-1 protein using 65288-1-Ig

    ELISA experiment of Mouse PD-1 protein using 65288-1-Ig

    ELISA test of 65288-1-Ig. Mouse PD-1 (Eg0918) was coated at 70 ng/well followed by blocking. Serial diluted Rat anti-Mouse PD-1 antibody 65288-1-Ig was appied and detected with HRP conjugated secondary antibody. Signal was developed by TMB substrate.

  • Neutralization experiment of CD279 (PD-1) using 65288-1-Ig

    Neutralization experiment of CD279 (PD-1) using 65288-1-Ig

    Mouse PD-L1 (Eg0985) was coated at 70 ng/well. Serial diluted HRP conjugated mouse PD-1 (Eg0918) was added for binding test (blue cureve, refer to bottom X - left Y). Blocking test was performed by mixing serial diluted Rat anti-mouse PD-1 antibody 65288-1-Ig with 1 ug/mL HRP conjugated mouse PD-1 (Eg0918) followed by same dose coated Mouse PD-L1 (Eg0985) . Typicall ND50 is around 1ug/mL.

  • Neutralization experiment of CD279 (PD-1) using 65288-1-Ig

    Neutralization experiment of CD279 (PD-1) using 65288-1-Ig

    Mouse PD-1 (Eg0918) was coated at 70 ng/well. Serial diluted HRP conjugated mouse PD-L1 (Eg0985) was added for binding test (blue cureve, refer to bottom X - left Y). Blocking test was performed by mixing serial diluted Rat anti-mouse PD-1 antibody 65288-1-Ig with 1 ug/mL HRP conjugated mouse PD-L1 (Eg0985) followed by applyting to 70 ng/well coated Mouse PD-1 (Eg0918) . Typicall ND50 is around 1-5 ug/mL.

Anti-Mouse PD-1/CD279 (RMP1-14)

PD-1/CD279 Monoclonal Antibody for Neutralization, ELISA
Cat No. 65288-1-Ig

产品说明书

CloneNo. RMP1-14

宿主/亚型

Rat / IgG2a, kappa

种属反应性

mouse

应用

Neutralization, ELISA

别名

CD279, PD1, PD-1, PD 1, Pdc1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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产品信息

65288-1-Ig targets PD-1/CD279 in Neutralization, ELISA applications and shows reactivity with mouse samples.

经测试应用 Neutralization, ELISA Application Description
经测试反应性 mouse
免疫原 Mouse PD-1 transfected BHK cells 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 programmed cell death 1
别名 CD279, PD1, PD-1, PD 1, Pdc1
GenBank蛋白编号BC119179
基因名称 PD-1
Gene ID (NCBI) 18566
RRIDAB_3084915
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). RMP1-14 is a monoclonal antibody that targets the murine PD-1 protein and can be used in mouse models to block the reaction between PD-1 and PD-L1/PD-L2 (PMID: 14764726; 31832706).