验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, mouse spleen tissue |
Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
32150-1-AP targets CD24 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | mouse |
免疫原 | CD24 fusion protein Eg2104 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | CD24a antigen |
别名 | Cd24a, CD24a antigen, HSA, Ly 52, nectadrin |
计算分子量 | 8kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | NM_009846.2 |
基因名称 | CD24 |
Gene ID (NCBI) | 12484 |
RRID | AB_3670209 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | P24807 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)
实验方案
Product Specific Protocols | |
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WB protocol for CD24 antibody 32150-1-AP | Download protocol |
IHC protocol for CD24 antibody 32150-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |