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验证数据展示

  • WB analysis using 32150-1-AP

    WB analysis using 32150-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32150-1-AP (CD24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 32150-1-AP

    IHC staining of mouse spleen using 32150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32150-1-AP (CD24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 32150-1-AP

    IHC staining of mouse spleen using 32150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32150-1-AP (CD24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD24 Polyclonal antibody

CD24 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32150-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Cd24a, CD24a antigen, HSA, Ly 52, nectadrin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32150-1-AP targets CD24 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2104

Product name: Recombinant Mouse CD24 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 27-53 aa of NM_009846.2

Sequence: NQTSVAPFPGNQNISASPNPSNATTRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD24a antigen
别名 Cd24a, CD24a antigen, HSA, Ly 52, nectadrin
计算分子量8 kDa
观测分子量50-55 kDa
GenBank蛋白编号NM_009846.2
基因名称 CD24
Gene ID (NCBI) 12484
RRIDAB_3670209
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP24807
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

实验方案

Product Specific Protocols
IHC protocol for CD24 antibody 32150-1-APDownload protocol
WB protocol for CD24 antibody 32150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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