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DARC Polyclonal antibody

DARC Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 55185-1-AP

Print datasheet

Synonyms

CCBP1, CD234, DARC, Dfy, FY, Fy glycoprotein, Glycoprotein D, GPD, GpFy, Plasmodium vivax receptor, WBCQ1

验证数据展示

  • WB analysis of K-562 using 55185-1-AP

    WB analysis of K-562 using 55185-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 55185-1-AP (DARC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55185-1-AP

    IHC staining of human kidney using 55185-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55185-1-AP (DARC antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 55185-1-AP

    IHC staining of human kidney using 55185-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55185-1-AP (DARC antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inK-562 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55185-1-AP targets DARC in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Duffy blood group, chemokine receptor
Synonyms CCBP1, CD234, DARC, Dfy, FY, Fy glycoprotein, Glycoprotein D, GPD, GpFy, Plasmodium vivax receptor, WBCQ1
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 33-35 kDa
GenBank Accession NumberNM_002036
Gene Symbol DARC
Gene ID (NCBI) 2532
RRIDAB_10838809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16570
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD234, also named as DARC, Duffy, FY and GPD, belongs to the G-protein coupled receptor Duffy family. It is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. This antibody is specific to CD234.

实验方案

Product Specific Protocols
WB protocol for DARC antibody 55185-1-APDownload protocol
IHC protocol for DARC antibody 55185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Ocul Surf

Single-cell RNA sequencing reveals the complex cellular niche of pterygium

Authors - Xueling Zhang
View Article