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验证数据展示

  • IHC staining of human kidney using 55185-1-AP

    IHC staining of human kidney using 55185-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 55185-1-AP (DARC antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of K-562 using 55185-1-AP

    WB analysis of K-562 using 55185-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 55185-1-AP (DARC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 55185-1-AP

    WB analysis using 55185-1-AP

    Human kidney tissue were subjected to SDS PAGE followed by western blot with 55185-1-AP (DARC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 55185-1-AP

    IHC staining of human colon using 55185-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55185-1-AP (DARC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DARC Polyclonal antibody

DARC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55185-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

ACKR1, Atypical chemokine receptor 1, CCBP1, CD234, Dfy

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman kidney tissue, K-562 cells
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55185-1-AP targets DARC in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Duffy blood group, chemokine receptor
别名 ACKR1, Atypical chemokine receptor 1, CCBP1, CD234, Dfy
计算分子量 36 kDa
观测分子量 33-35 kDa
GenBank蛋白编号NM_002036
基因名称 DARC
Gene ID (NCBI) 2532
RRIDAB_10838809
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16570
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD234, also named as DARC, Duffy, FY and GPD, belongs to the G-protein coupled receptor Duffy family. It is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. This antibody is specific to CD234.

实验方案

Product Specific Protocols
IHC protocol for DARC antibody 55185-1-APDownload protocol
WB protocol for DARC antibody 55185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IF

Ocul Surf

Single-cell RNA sequencing reveals the complex cellular niche of pterygium

Authors - Xueling Zhang
View Article
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