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Anti-Mouse CD18 (M18/2)

CD18 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

M18/2

Cat No : 65178-1-Ig

Print datasheet

Synonyms

2E6, AI528527, Cd18, integrin beta 2, Itgb2, LAD, LCAMB, Lfa1, MF17

验证数据展示

  • FC experiment of BALB/c mouse thymocytes using 65178-1-Ig

    FC experiment of BALB/c mouse thymocytes using 65178-1-Ig

    1X10^6 BALB/c mouse thymocytes were surface stained with 1 ug Anti-Mouse CD18 (65178-1-Ig, Clone:M18/2) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 1 ug Control Antibody. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse thymocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65178-1-Ig targets CD18 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen Mouse CTL glycoprotein 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name integrin beta 2
Synonyms 2E6, AI528527, Cd18, integrin beta 2, Itgb2, LAD, LCAMB, Lfa1, MF17
GenBank Accession NumberBC145644
Gene Symbol Itgb2
Gene ID (NCBI) 16414
RRIDAB_2918453
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP11835
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

实验方案

Product Specific Protocols
FC protocol for CD18 antibody 65178-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols