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验证数据展示

  • WB analysis using 33091-1-AP

    WB analysis using 33091-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33091-1-AP (CD146/MCAM antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 33091-1-AP

    IHC staining of mouse liver using 33091-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 33091-1-AP (CD146/MCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 33091-1-AP

    IHC staining of mouse liver using 33091-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 33091-1-AP (CD146/MCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33091-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inbEnd.3 cells, mouse lung tissue, rat lung tissue
Positive IHC detected inmouse liver tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33091-1-AP targets CD146/MCAM in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原

CatNo: Eg2667

Product name: Recombinant Mouse CD146/MCAM protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-563 aa of NM_023061.2

Sequence: VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 melanoma cell adhesion molecule
别名 CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule
计算分子量72 kDa
观测分子量120 kDa
GenBank蛋白编号NM_023061.2
基因名称 Mcam
Gene ID (NCBI) 84004
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8R2Y2-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as a marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

实验方案

Product Specific Protocols
IHC protocol for CD146/MCAM antibody 33091-1-APDownload protocol
WB protocol for CD146/MCAM antibody 33091-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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