验证数据展示
经过测试的应用
Positive WB detected in | MJ cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
31805-1-AP targets CD134/OX40 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | tumor necrosis factor receptor superfamily, member 4 |
别名 | TNFRSF4, TAX transcriptionally-activated glycoprotein 1 receptor, OX40L receptor, OX40, CD134 |
计算分子量 | 29 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | NM_003327 |
基因名称 | CD134 |
Gene ID (NCBI) | 7293 |
RRID | AB_3670120 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | P43489 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.
实验方案
Product Specific Protocols | |
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WB protocol for CD134/OX40 antibody 31805-1-AP | Download protocol |
IHC protocol for CD134/OX40 antibody 31805-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |