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验证数据展示

  • WB analysis using 29361-1-AP

    WB analysis using 29361-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29361-1-AP (CD134/OX40 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

CD134/OX40 Polyclonal antibody

CD134/OX40 Polyclonal Antibody for WB, ELISA
Cat No. 29361-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

ACT35, ACT35 antigen, CD134, CD134/OX40, OX40, OX40L receptor, TNFRSF4, TXGP1L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMJ cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29361-1-AP targets CD134/OX40 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 CD134/OX40 fusion protein Ag28521 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor necrosis factor receptor superfamily, member 4
别名 ACT35, ACT35 antigen, CD134, CD134/OX40, OX40, OX40L receptor, TNFRSF4, TXGP1L
计算分子量 29 kDa
观测分子量50 kDa
GenBank蛋白编号NM_003327
基因名称 CD134
Gene ID (NCBI) 7293
RRIDAB_3669684
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43489
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD134, also known as OX40 and TNFRSF4, is a member of the TNFR-superfamily of receptors (PMID: 2828930; 9766631). It is a type I transmembrane protein predominantly expressed on activated T cells which include CD4 and CD8 T cells, Th2, Th1, and Th17 cells, as well as regulatory T cells (Tregs) (PMID: 20307208). CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule (PMID: 26215166). CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmune diseases, cancer and infectious disease (PMID: 26215166; 19426222).

实验方案

Product Specific Protocols
WB protocol for CD134/OX40 antibody 29361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols