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验证数据展示

  • WB analysis using 32752-1-AP

    WB analysis using 32752-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32752-1-AP (CD13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 32752-1-AP

    IHC staining of mouse spleen using 32752-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32752-1-AP (CD13 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD13 Polyclonal antibody

CD13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32752-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Anpep, AP-M

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse kidney tissue, mouse liver tissue, mouse small intestine tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32752-1-AP targets CD13 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 CD13 fusion protein Eg3592 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alanyl (membrane) aminopeptidase
别名 Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Anpep, AP-M
计算分子量110 kDa
观测分子量150 kDa
GenBank蛋白编号NM_008486.2
基因名称 Anpep
Gene ID (NCBI) 16790
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP97449
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD13, also named as APN, ANPEP(aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

实验方案

Product Specific Protocols
WB protocol for CD13 antibody 32752-1-APDownload protocol
IHC protocol for CD13 antibody 32752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols