验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
65518-1-PBS targets CD11a in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 |
HLA-DR CTL line 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) |
| 别名 | CD11 antigen-like family member A, Integrin alpha L, Integrin alpha-L, ITGAL, Leukocyte adhesion glycoprotein LFA-1 alpha chain |
| 计算分子量 | 129 kDa |
| GenBank蛋白编号 | BC008777 |
| 基因名称 | CD11a |
| Gene ID (NCBI) | 3683 |
| ENSEMBL Gene ID | ENSG00000005844 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P20701 |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. Stable for one year after shipment. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD11a antibody 65518-1-PBS | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
