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验证数据展示

  • WB analysis using 87060-4-RR

    WB analysis using 87060-4-RR

    LPS, PMA, and Brefeldin A-treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 87060-4-RR (CCL5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 87060-4-RR

    IHC staining of mouse spleen using 87060-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87060-4-RR (CCL5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 87060-4-RR

    IHC staining of mouse spleen using 87060-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87060-4-RR (CCL5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CCL5 Recombinant monoclonal antibody

CCL5 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 87060-4-RR

产品说明书

CloneNo. 250841C2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

C-C motif chemokine 5, MuRantes, RANTES, Scya5, SIS-delta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPMA, LPS and Brefeldin A treated RAW 264.7 cells
Positive IHC detected inmouse spleen tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87060-4-RR targets CCL5 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2253

Product name: Recombinant Mouse CCL5 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 24-91 aa of Q5XZF2

Sequence: SPYGSDTTPCCFAYLSLALPRAHVKEYFYTSSKCSNLAVVFVTRRNRQVCANPEKKWVQEYINYLEMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-C motif) ligand 5
别名 C-C motif chemokine 5, MuRantes, RANTES, Scya5, SIS-delta
计算分子量10kd
观测分子量8 kDa
GenBank蛋白编号Q5XZF2
基因名称 Ccl5
Gene ID (NCBI) 20304
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30882
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCL5 (C-C motif chemokine ligand 5), also known as RANTES, is a key inflammatory chemokine that attracts immune cells like T cells, monocytes, and eosinophils to sites of infection or inflammation, playing vital roles in immunity, viral defense (including HIV), and sometimes cancer progression, by binding to receptors like CCR1, CCR3, and CCR5 to signal cell movement and activation, with inhibitors being researched for therapeutic use.

实验方案

Product Specific Protocols
IHC protocol for CCL5 antibody 87060-4-RRDownload protocol
WB protocol for CCL5 antibody 87060-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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