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验证数据展示

  • IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

    IHC staining of human colon cancer using 18214-1-AP (same clone as 18214-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18214-1-AP (CCL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

  • WB analysis of human spleen using 18214-1-AP (same clone as 18214-1-PBS)

    WB analysis of human spleen using 18214-1-AP (same clone as 18214-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 18214-1-AP (CCL28 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18214-1-PBS in a different storage buffer formulation.

CCL28 Polyclonal antibody, PBS Only

CCL28 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18214-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

18214-1-PBS targets CCL28 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 CCL28 fusion protein Ag12920 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 28
别名
计算分子量 127 aa, 14 kDa
观测分子量 8 kDa-9 kDa
GenBank蛋白编号BC069532
基因名称 CCL28
Gene ID (NCBI) 56477
RRIDAB_2262251
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRJ3
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCL28, also named as SCYA28, CCK1 and MEC, belongs to the intercrine beta (chemokine CC) family. It has chemotactic activity for resting CD4, CD8 T-cells and eosinophils. CCL28 binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. It is produced by epithelial cells of these tissues suggesting that this chemokine can play an important role by linking homing mechanisms between the gut, nasal mucosa and mammary gland (MG). In WB test, CCL28 is 14kd and 8-9kd(a putatively to a degradation fragment).