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验证数据展示

  • WB analysis of mouse testis using 20396-1-AP

    WB analysis of mouse testis using 20396-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20396-1-AP (CCDC158 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 20396-1-AP

    IHC staining of human kidney using 20396-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 20396-1-AP

    IHC staining of human kidney using 20396-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20396-1-AP (CCDC158 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse testis using 20396-1-AP

    IP experiment of mouse testis using 20396-1-AP

    IP result of anti-CCDC158 (IP:20396-1-AP, 4ug; Detection:20396-1-AP 1:500) with mouse testis tissue lysate 6000ug.

CCDC158 Polyclonal antibody

CCDC158 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 20396-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA and More (1)

别名

CCDC158

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20396-1-AP targets CCDC158 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 CCDC158 fusion protein Ag14049 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 158
别名 CCDC158
计算分子量 1113 aa, 127 kDa
观测分子量 127-140 kDa
GenBank蛋白编号BC035224
基因名称 CCDC158
Gene ID (NCBI) 339965
RRIDAB_10694278
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5M9N0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CCDC158 antibody 20396-1-APDownload protocol
IHC protocol for CCDC158 antibody 20396-1-APDownload protocol
IP protocol for CCDC158 antibody 20396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

J Cell Physiol

CCDC158: A novel regulator in renal proximal tubular endocytosis unveiled through exome sequencing and interactome analysis

Authors - Tjessa Bondue
View Article